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nebuffer r1 1 (New England Biolabs)

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Structured Review

New England Biolabs nebuffer r1 1
Nebuffer R1 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuffer+r1+1/NEBuffer+r2%2E1/pm42009660-478-17-19
Average 99 stars, based on 506 article reviews

nebuffer r1 1 - by Bioz Stars, 2026-09

99/100 stars

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Activity Assay:

Article Title: Sensitive detection of FPG based on 8-oxoG modified chimeric peptide-DNA enzyme for oxidative damage evaluation.
Article Snippet: Formamidopyrimidine DNA glycosylase (FPG) is a crucial DNA repair enzyme that specifically recognizes and excises the damaged base 7,8-dihydro-8-oxoguanine (8-oxoG).. The current detection technology for FPG is limited due to the need of integrating the relatively independent identification components and signal amplifiers.. Herein, we designed an integrated probe (loaded on magnetic beads), which contained 8-oxoG for FPG recognition and a novel chimeric peptide-DNA mimetic enzyme (CPDzyme) for chemiluminescence (CL) signal amplification.

Incubation:

Article Title: DNA deformability in sequence-dependent capture of E. coli gyrase.
Article Snippet: An aliquot was analyzed using an Agilent TapeStation (D5000 ScreenTape) to confirm that gyrase protects fragments of the DNA from digestion by micrococcal nuclease. .. Samples of the gyrase protected DNA were incubated with different restriction enzymes in either NEBuffer r2.1 or NEBuffer r1.1 (NEB). .. Samples of the gyrase protected DNA were incubated with different restriction enzymes in either NEBuffer r2.1 or NEBuffer r1.1 (NEB).

Concentration Assay:

Article Title: Benchmarking of duplex sequencing approaches to reveal somatic mutation landscapes
Article Snippet: DNA was randomly fragmented to a peak size of ∼4 kb using a Megaruptor 3 (Diagenode) fitted with short hydropores (cat. # E07010001) in a volume of 100 μL with 3 consecutive runs at speed ‘65’. .. DNA overhangs were blunted by incubating the DNA with Nuclease P1 (NEB) at 0.5 U/μL (buffer: NEBuffer r1.1) for 30 mins at 37 °C followed by inactivation with a final concentration of 19 mM EDTA and 0.01 % SDS and a cleanup with Ampure PB beads (PacBio) at a 1.5X bead to volume ratio. .. DNA was then treated with T4 PNK (NEB) at 0.4 U/μL (buffer: rCutSmart; 4mM DTT; 1mM ATP) for 60 mins at 37 °C.